Your Question :-

How to analyse UV inactive compound on HPLC using UV Detector?

In my research field I got UV inactive compound having one Chiral Center I want to separate the enantiomers on HPLC having UV detector So what can i do to get UV response on HPLC. ?

Answer - 

I suggest to complex the compound with some kind of regents to give a color so you can detect in visible range. For the enantiomer you can modify a mobile phase containing 5% beta-cyclodextrine to enhance selectivity towards enentiomers.Derivatization is one way, but more laborious and expensive. You can try the more straightforward approach of indirect UV detection.  The detection of a UV-Vis-transparent analyte is accomplished by adding light-absorbing species into the mobile phase. The presence of the analyte is monitored by measuring a decrease or The only way you could analyze a UV inactive compound on a UV detector is to derivatize it to add a UV active functionality, such as a phenyl ring.  Otherwise you can't use UV on the compounds in their native state since there is nothing for the UV detector to detect. If you are doing this as a preparative LC than derivatization wouldn't be an option.  Your only recourse would be to use another type of detector.